Template Switch Oligo - Web learn how to use the template switching rt enzyme mix (neb #m0466) to synthesize and amplify cdna from rna. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse. Web template switching is believed to happen in a sequential process involving nontemplated addition of three protruding nucleotides. Web learn how to identify the 5′ end of an rna transcript using template switching reverse transcription and pcr.
Web learn how to identify the 5′ end of an rna transcript using template switching reverse transcription and pcr. Web learn how to use the template switching rt enzyme mix (neb #m0466) to synthesize and amplify cdna from rna. Web template switching is believed to happen in a sequential process involving nontemplated addition of three protruding nucleotides. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse.